WebThe following most common reagents have been tested and validated by the CAMAG laboratory for use with the Derivatizer: Sulfuric acid reagent (10 % in methanol) Anisaldehyde reagent; Natural product reagent; Polyethylene glycol solution; Iodine solution (0.5% in ethanol) Dragendorff reagent; Fast blue salt B reagent; Ehrlich’s reagent WebAug 1, 2024 · Cells that are “acid-fast” because of the mycolic acid in their cell wall resist decolorization and retain the primary stain. All other cell types will be decolorized. Methylene blue is then used as a counterstain. In the end, acid-fast bacteria (AFB) will be stained a bright pink color, and all other cell types will appear blue.
CAMAG® Derivatizer CAMAG
WebApr 14, 2024 · The Acid-Fast staining basically requires 3 reagents, A carbol Fuchsin Solution, An Acid Fast decolorizer and The Counter Stain, Methylene blue is commonly used. In this article, I’m gonna discuss the Preparation of All the Reagents require for the Acid Fast staining, in Laboratory. Let’s Start with Carbol Fuchsin preparation http://www.cantonhealth.org/pdf/400-001-06-13-A_API%20NH%20System%20BioMerieux%20Product%20Insert.pdf ibm corporation 3039 cornwallis road
Fast Blue Substrate (20 mL Blue Buffer + 420 uL each …
WebFast blue salt BB [18] or fast blue salt RR [18,19] can be employed in the reagent in place of fast blue salt B. It is occasionally preferable not to apply spray solutions I and II separately but to work directly with a 0.1% solution of fast blue salt B in caustic soda solution (c=l—2mol/l) [13, 15] or in 0.5% methanolic caustic potash [3]. WebApplication: Fast blue BB is a dye with Color Index Number: 37175. CAS Number: 120-00-3. Molecular Weight: 300.36. Molecular Formula: C 17 H 20 N 2 O 3. For Research Use Only. Not Intended for Diagnostic or Therapeutic Use. * Refer to Certificate of Analysis for lot specific data (including water content). RECEIVE –15–CRUZ CREDITS™. WebSubsequent use of methylene blue will color only the non-acid-fast cells because methylene blue cannot penetrate the waxy cell wall of the acid-fast cells. This leaves the acid-fast cells colorless, and the non-acid-fast cells blue. Arrange the steps of the acid-fast procedure in the correct order. 1. Place slide over heated beaker of water. 2. ibm corporate wellness